Quantitation of amoxicillin in urine by nuclear magnetic resonance. application to five cases

Abstract

Aim In this paper, we propose a procedure for the analysis and quantification of amoxicillin in urine with minimum pre-treatment of the samples using Nuclear Magnetic Resonance spectroscopy (NMR). Five clinical samples were analyzed and amoxicillin was detected and quantified in each case.Methods Samples of urine from amoxicillin users were collected in accordance with an IRB-approved protocol. Donors were asked to record the day of urine collection, the length of treatment and the amount of drug prescribed. Samples were collected and immediately frozen at -20°C until required for experiments. The water suppression technique “Watersculpt” was used in all
samples. A rapid acidic pre-treatment eliminated the urea signal from the NMR spectra of urine samples. A calibration curve was obtained using spiked samples and by integration of selected signals from amoxicillin. The method gave a linear response (correlation coefficient of 0.995) over the range 0.011 mg/mL.Results Subsequent quantitation of the amount of amoxicillin present in samples from clinical cases was performed. The concentration of the drug in urine varied from 0.517 mg/mL to 0.326 mg/mL.ConclusionThe benefit and reliability of NMR investigations of human urine for the detection of amoxicillin are discussed.

Keywords

Additional Information

Publisher’s Note
Bayrakol MP remains neutral with regard to jurisdictional and institutional claims.

Rights and Permissions

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). To view a copy of the license, visit https://creativecommons.org/licenses/by-nc/4.0/

View full license details →